Effects of Ethylenediaminetetraacetate and Metal Ions in Intestinal Absorption of Vitamin B12 in Man and Rats

Abstract
Summary The effect of metal chelation on intestinal adsorption of B12 was studied in man and in rats. When a physiological dose of B12-Co60 was instilled through an indwelling tube into the duodenum in man, immediately followed by 2 g of EDTA in a dilute solution, absorption was reduced markedly as studied by the Schilling test. Administered shortly after EDTA, Ca++, Mg++, Sr++, but not Fe++, Fe+++, or Zn++ counteracted the inhibition by EDTA. Similar results were obtained with rat intestinal loops. When there was interval between the dosage of B12-Co60 and EDTA, about 3 hours in man and 45 minutes in the case of rat loop were necessary for the absorption to become unaffected by EDTA.

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