High-Performance Liquid Chromatographic Determination of Zinc Pyrithione in Antidandruff Shampoos Using On-Line Copper Chelate Formation

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Abstract
A rapid and simple high-performance liquid chromatographic (HPLC) method using on-line copper chelate formation was developed for the determination of zinc pyrithione (ZPT) in antidandruff shampoos. ZPT dispersed In samples was dissolved in 1 M hydrochloric acid followed by purification with polyamide microcolumn. HPLC was carried out on a Chromatorex ODS column (25 cm × 4.6 mm I. D.) using 15 % methanol containing 1 % of copper (II) nitrate trihydrate and 1% of citric acid monohydrate as the mobile phase with UV detection at 320 nm. The calibration graph was linear from 20-50 ng for ZPT. The method gave high reproducibility with coefficients of variation less than 1 %.