Anaerobic Coryneforms Isolated from Human Bone Marrow and Skin

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Abstract
Isolates of anaerobic coryneforms (18) from human bone marrow and skin 4 type strains of Propionibacterium were studied chemically, biochemically and antigenically. All isolates were identified as P. acnes; of the 18 isolates, 16 belonged to serotype I and 2 to serotype II. By GLC and mass spectral analysis, a large amount of iso-type fatty acids, i.e., iso-pentadecanoic and iso-heptadecanoic acids, were detected in whole cells of isolates and type strains. Antitumor and adjuvant effects of the isolates and type strains differed considerably among the strains. One isolate, P. acnes C-7, which showed potent biological activities, was fractionated by hog phenol-water extraction. The resulting insoluble middle layer was the most effective in tumor protection, adjuvant action in immune response and phagocytic activity in mice.