Effect of hypothyroidism and thyroid hormone replacement on the level of protein kinase C and protein kinase A in rat liver
- 6 May 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 282 (2) , 397-400
- https://doi.org/10.1016/0014-5793(91)80522-5
Abstract
We investigated the influence of the thyroid hormone status on the levels of protein kinase C (PKC) and A (PKA) in the soluble fraction of rat liver. The immunodetectable PKC level in hypothyroid liver was elevated 7.7-fold, whereas the phorbol-ester binding capacity and the immunodetectable α-PKC level were increased 2.4- and 2.6-fold, respectively. Conversely, in hypothyroid livers the abundance of the regulatory type I and the catalytic subunits of PKA were lowered to 42% of the euthyroid level as determined by immonoblotting and by measuring the substrate specific phosphorylation rate of PKA. These changes in the PKC and PKA levels were reversible upon treatment with 0.5 μg T4/100 g body weight for 2–21 days. The thyroid state dependent alterations in hepatic PKC and PKA levels may be responsible for the Known changes in the response of hepatocytes to other hormonal stimuli in hypothyroidism.Keywords
This publication has 19 references indexed in Scilit:
- Protein kinase C in transmembrane signallingFEBS Letters, 1990
- Regulation of Gene Expression by Thyroid HormoneAnnual Review of Physiology, 1989
- Permissive hormone regulation of hormone-sensitive effector systemsTrends in Pharmacological Sciences, 1988
- Immunological quantitation of phospholipid/CA2+‐dependent protein kinase of human mammary carcinoma cells: Inverse relationship to estrogen receptorsInternational Journal of Cancer, 1987
- PROTEIN SERINE/THREONINE KINASESAnnual Review of Biochemistry, 1987
- cAMP mediated proteolysis of the catalytic subunit of cAMP‐dependent protein kinaseFEBS Letters, 1986
- Effect of hypothyroidism on the cytosolic free Ca2+ concentration in rat hepatocytes during rest and following stimulation by noradrenaline or vasopressinBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- The cytosolic phorboid receptor correlates with hormone dependency in six mammary carcinoma cell linesBiochemical and Biophysical Research Communications, 1985
- Thyroid Hormone Increases Type I Adenosine 3′,5′- Monophosphate-Dependent Protein Kinase and Casein Kinase Activities in Rat Liver Cytosol: Analysis of Protein Kinases by Polyacrylamide Disc Gel Electrophoresis*Endocrinology, 1983
- Thyroid hormone attenuates and augments hepatic gene expression at a pretranslational level.Proceedings of the National Academy of Sciences, 1981