Genistein Inhibits Ca2+Influx by Extracellular ATP in PC12 Pheochromocytoma Cells
- 1 June 1995
- journal article
- research article
- Published by Georg Thieme Verlag KG in Hormone and Metabolic Research
- Vol. 27 (06) , 272-274
- https://doi.org/10.1055/s-2007-979957
Abstract
We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 µg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.Keywords
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