Mode of action of yeast toxins: energy requirement for Saccharomyces cerevisiae killer toxin

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Abstract
The role of the energy status of the yeast cell in the sensitivity of cultures to 2 yeast toxins was examined by using 42K release from cells as a measure of toxin action. The S. cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy, but it was unable to efflux 42K from the cells under these conditions. In contrast, the Torulopsis glabrata pool efflux-stimulating toxin-induced efflux of the yeast 42K pool was insensitive to the presence of energy poisons in cultures. The results indicate that an energized state, maintained at the expense of ATP from either glycolytic or mitochondrial reactions, is required for the action of the killer toxin on the yeast cell.