Hemoglobin A1C by Hplc with the Pharmacia Mono S HR 5/N Cation-Exchange Column: Influence of Sample Protein Load on Optimal Chromatographic Conditions
Open Access
- 1 August 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 38 (8) , 1488-1490
- https://doi.org/10.1093/clinchem/38.8.1488
Abstract
The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.Keywords
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