Tryptophan Micro-Scale Determinations by Rapid Hydrolysis
- 1 January 1984
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 365 (2) , 1183-1186
- https://doi.org/10.1515/bchm2.1984.365.2.1183
Abstract
A rapid acid-hydrolysis micromethod was developed for the accurate determination of the tryptophan contents of proteins. Sample protein (5 .mu.g) was hydrolyzed in 30 .mu.l of 3 M mercaptoethanesulfonic acid at 166.degree. C for 25 min, resulting in total hydrolysis of the peptide bonds. The subjection of the hydrolysate to an amino-acid analyzer revealed the amino-acid composition, including the content of tryptophan with a high recovery of 92%.This publication has 4 references indexed in Scilit:
- A new rapid method for acid hydrolysis of protein.Proceedings of the Japan Academy, Series B, 1982
- High recovery of tryptophan from acid hydrolysates of proteinsBiochemical and Biophysical Research Communications, 1969