An inducible iron-containing superoxide dismutase in Rhizobium japonicum
- 1 November 1981
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 27 (11) , 1202-1208
- https://doi.org/10.1139/m81-185
Abstract
Crude extracts of Rhizobium japonicum contained a single superoxide dismutase (SOD) as revealed by polyacrylamide gel electrophoresis. Rhizobium japonicum SOD was determined to be iron-containing by criteria of sensitivity to 5 mM H2O2 and 1 mM NaN3. Growth media strongly influenced SOD activity. The addition of methyl viologen to cultures of R. japonicum 61A76NS, growing in complex medium, caused induction of the enzyme. Yeast extract was essential for induction of SOD. Puromycin blocked the induction of the enzyme. Rhizobium spp. were surveyed for SOD activity, electrophoretic mobility on polyacrylamide gels, and metal content.Keywords
This publication has 2 references indexed in Scilit:
- Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilizationApplied and Environmental Microbiology, 1976
- Superoxide dismutase: Improved assays and an assay applicable to acrylamide gelsAnalytical Biochemistry, 1971