GENE UNSTABILIZATION INDUCED BY HEAT AND BY NITROUS ACID
- 1 January 1961
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 81 (1) , 111-+
- https://doi.org/10.1128/jb.81.1.111-117.1961
Abstract
Treatment by heat (130 to 155 C) or by nitrous acid induces stable mutants and also unstable lactose-fermentation mutants in E. coli and unstable auxotrophs in both E- coli and B. subtilis. The unstable fermentation mutants were isolated from some of the sectored colonies on a medium (Penassay-tetrazolium-lactose agar) which also permits detection of mutants with low levels of [beta]-galactosidase. The back-mutation rates of unstable auxtrophs were 10-4 to 4 x 10-2. The unstable strains can be maintained indefinitely.Keywords
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