Synthesis of a mammalian parvovirus in Brij-58-lysed cells

Abstract
An in vitro system was developed, prepared by lysis of parvovirus LuIII-infected [newborn human kidney NB cells] cells with Brij-58. In this subcellular system, DNA of viral genome size was synthesized and subsequently packaged into particles with physicochemical properties of mature LuIII virions.