Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus
- 1 December 1985
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 50 (3) , 922-924
- https://doi.org/10.1128/iai.50.3.922-924.1985
Abstract
Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in the isolation of a 3.2-kilobase DNA fragment containing the cytotoxin gene. By using this fragment as a DNA probe, homologous gene sequences were detected in all 54 V. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.This publication has 19 references indexed in Scilit:
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