A Tannic Acid Modified Hemagglutination Test for Infectious Bronchitis of Chickens

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Abstract
An indirect hemagglutination test for detecting infectious bronchitis virus antibodies in chicken serum is described. Of 20 sera negative to the SN test, 19 yielded titers of 1:8 or lower. Of 55 sera possessing in ovo serum neutralization antibodies, 52 gave serum HA titers exceeding 1:8. These titers ranged from 1:16 to 1:1024. In negative sera appearing positive to the hemagglutination test when irregularities were noted in the serum-RBC control tube, adsorption of the sera with normal horse RBC removed the interfering factor(s). The test was more sensitive at 4[degree]C than at room temperature (22-26[degree]C) and the readings were stable for 3-4 days. Two virus strains and cells from 3 different horses were equally effective in this study.