Human liver mitochondrial aldehyde dehydrogenase: a C‐terminal segment positions and defines the structure corresponding to the one reported to differ in the Oriental enzyme variant

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Abstract
A C-terminal segment of mitochondrial human liver aldehyde dehydrogenase was characterized. The results prove that a central part of this segment largely but not completely agrees with a structure of a tryptic peptide previously reported for the same isoenzyme. This part corresponds to a segment that contains the exchanged residue in the functionally deficient Oriental variant of mitochondrial aldehyde dehydrogenase [(1984) Proc. Natl. Acad. Sci. USA 81, 258-261]. The data suggest important functions for the C-terminal region of aldehyde dehydrogenase, clarify previously inconsistent results, and establish this structure in the typical enzyme, including the position corresponding to the mutation in the functional variant.Mitochondrial isoenzymeAmino acid sequenceIsoenzyme differenceStructure-function relationshi