Interactions of norharman and harman with DNA
- 1 November 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (11) , 3679-3686
- https://doi.org/10.1093/nar/4.11.3679
Abstract
The interactions of norharman (9H-pyrido[3,4-b]indole) and harman (1-methyl-9H-pyrido[3,4-b]indolel with DNA were studied. DNA caused remarkable fluorescence quenching and change in the absorption spectra of the dyes. Scatchard plots obtained by optical titration gave Kd values of 2.2 × 10 −5 M and 7.7 × 10 −6 M, and apparent numbers of binding sites of 0.13/base and 0.12/base for norharman and harman, respectively. Agarose gel electro-phoresis of circular DNA, closed in the presence or absence of norharman revealed that the dye intercalates DNA, thereby causing 17 ± 3° unwinding of the double helix.Keywords
This publication has 5 references indexed in Scilit:
- The number of superhelical turns in native Virion SV40 DNA and Minicol DNA determined by the band counting methodCell, 1976
- Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.Proceedings of the National Academy of Sciences, 1975
- Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.Proceedings of the National Academy of Sciences, 1975
- Conformational fluctuations of DNA helix.Proceedings of the National Academy of Sciences, 1975
- The degree of unwinding of the DNA helix by ethidiumJournal of Molecular Biology, 1974