ENDOCRINE RESPONSIVENESS IN HUMAN MELANOCYTES AND MELANOMA-CELLS IN CULTURE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 66  (5) , 799-802
Abstract
Studies were performed for the investigation of endocrine responsiveness in cell lines derived from normal human melanocytes or human melanoma cells. Alterations in differentiation (tyrosinase activity) were determined in cells exposed to melanocyte-stimulating hormone (MSH, 10-7 M), theophylline (10-3 M), N6,O2''-dibutyryl cAMP (db-cAMP, 10-4 M) or prostaglandin E1 (PGE1, 10-6 M). Cultures derived from normal uveal melanocytes demonstrated increased tyrosinase activity upon exposure to either theophylline, db-cAMP or PGE1, but not to MSH. MSH responsiveness was detected in 7 of 11 human melanoma cell lines. Four cell lines demonstrated increased activity of tyrosinase after MSH treatment; 3 lines showed an MSH-induced inhibition of enzyme activity. PGE1 was effective in stimulating tyrosinase activity in 5 of 9 cell lines examined. Theophylline was the most effective stimulator of tyrosinase in melanoma-derived cell populations and caused increased enzyme activity in 8 of 11 cell lines.