TARGET-CELLS OF HUMAN ADENOVIRUS-TYPE-12 IN SUBTENTORIAL BRAIN-TISSUE OF NEWBORN MICE .1. CYTO-HISTOMORPHOLOGIC AND IMMUNOFLUORESCENT MICROSCOPIC STUDIES INVIVO

  • 1 January 1976
    • journal article
    • research article
    • Vol. 30  (3) , 163-179
Abstract
Human adenovirus type 12 (Ad 12) was inoculated subtentorially into inbred newborn mice (C3H/BifB/Ki), and sequential changes of the brain and tumor induction were examined by histological and immunofluorescent methods. Two days after virus inoculation, Ad 12 specific tumor antigen (fluorescent T-antigen) appeared in the cells of ependymal and subventricular matrix layers, choroid plexuses and leptomeninges in the subtentorial as well as the supratentorial brain. After 10 days, these fluorescent positive cells decreased gradually in number but remained focally beneath the ependyma. Sixty days later, early tumor nodules were detected in the same regions in which remained the fluorescent cells. After 107 days, neurological signs and well developed tumors were noted in 25 of 83 (30.1%) mice examined. In the cerebellum, T-antigens and tumors were limited to the IVth ventricle, but not the granular layers. Histomorphologically, the tumors were of primitive neuroectodermal origin and consisted of cells resembling immature matrix cells in the subventricular zone. The virus has a possible selective affinity to the remaining matrix cells, but not cerebellar granular cells, at least, in newborn mice.

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