Chicken microchromosomes are hypermethylated and can be identified by specific painting probes
- 1 July 2001
- journal article
- animal cytogenetics-and-comparative-mapping
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 93 (3-4) , 265-269
- https://doi.org/10.1159/000056996
Abstract
Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes.Keywords
This publication has 3 references indexed in Scilit:
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