Dengue virus induced polypeptide synthesis

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Abstract
Dengue 2 virus polypeptide synthesis was investigated in BHK21 cells. Nine or ten virus induced polypeptides were identified, three of which are glycoproteins as demonstrated by tunicamycin treatment of infected cells. We performed pulse chase experiments, experiments with amino-acid analogs, protease inhibitors or pactamycin treatment of infected cells, to determine whether or not large polypeptide processing occurs. In some of these experiments a large polypeptide (P130) was immunoprecipitated by an anti-dengue 2 serum. We observed a transfer of label between small molecular weight polypeptides which might be the result of restricted proteolytic cleavage.