Continuous Monitoring ofin VztroOxidation of Human Low Density Lipoprotein
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 6 (1) , 67-75
- https://doi.org/10.3109/10715768909073429
Abstract
The kinetics of the oxidation of human low densit) lipoprotein (LDL) can be measured continuously by monitoring the change of the 234 nm diene absorption. The time-course shows three consecutive phases, a lag-phase during which the diene absorption increases only weakly. a propagation phase with a rapid increase of the diene absorption and finally a decomposition phase. The increase of the dienes is highly correlated with the increase of MDA or lipid hydroperoxides. The duration of the lag-phase is determined by the endogenous antioxidants contained in LDL (vitamin E. carotenoids. retinylstearate). Water-soluble antioxidants (ascorbic acid. urate) added in micromolar concentrations prolong the lag-phase in a concentration-dependent manner. The determination of the lag-phase is a convenient and objective procedure for determining the susceptibility of LDL from different donors towards oxidation as well as effects of pro-and antioxidants.Keywords
This publication has 21 references indexed in Scilit:
- Modification of human serum low density lipoprotein by oxidation — Characterization and pathophysiological implicationsChemistry and Physics of Lipids, 1987
- Oxidatively modified low density lipoproteins: a potential role in recruitment and retention of monocyte/macrophages during atherogenesis.Proceedings of the National Academy of Sciences, 1987
- Decrease in reactive amino groups during oxidation or endothelial cell modification of LDL. Correlation with changes in receptor-mediated catabolism.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1987
- Free radical modification of low-density lipoprotein: Mechanisms and biological consequencesFree Radical Biology & Medicine, 1987
- Oxidized low density lipoprotein induces ceroid accumulation by murine peritoneal macrophages in vitroAtherosclerosis, 1986
- Superoxide-mediated modification of low density lipoprotein by arterial smooth muscle cells.Journal of Clinical Investigation, 1986
- Monocytes and Neutrophils Oxidize Low Density Lipoprotein Making It CytotoxicJournal of Leukocyte Biology, 1985
- Endothelial and smooth muscle cells alter low density lipoprotein in vitro by free radical oxidation.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1984
- Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids.Proceedings of the National Academy of Sciences, 1984
- Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: recognition by receptors for acetylated low density lipoproteins.Proceedings of the National Academy of Sciences, 1981