Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta
Open Access
- 1 October 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (10) , 1903-1910
- https://doi.org/10.1093/nar/2.10.1903
Abstract
A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacryla-mide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,000 daltons similar to the active hormone. The messenger RNA activity for HPL corresponded to a sedimentation coefficient of 11–12 S. Furthermore the messenger activity for HPL was preferentially associated with membrane bound polyribosomes than with free polyribosomes.Keywords
This publication has 16 references indexed in Scilit:
- The effectiveness of human placental lactogen measurements as an adjunct in decreasing perinatal deaths. Results of a retrospective and a randomized controlled prospective study.1975
- The translation of a human placental lactogen mRNA fraction in heterologous cell-free systems: The synthesis of a possible precursorBiochemical and Biophysical Research Communications, 1975
- The synthesis of human placental lactogen by human placental ribosomes.1974
- Protein synthesis in a cell-free system prepared from human placenta II. pH 5 enzyme inefficiency due to defects in tRNA charging with resulting loss of elongation factor 1Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acidBiochemistry, 1974
- Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Localization in polysomes, induction by estrogen, and effect of actinomycin D.1973
- The Molecular Properties of Human Chorionic SomatomammotropinJournal of Biological Chemistry, 1971
- A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites CellsProceedings of the National Academy of Sciences, 1971
- Isolation and properties of polysomes from human placentaBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- Serum Placental Lactogen (HPL) Levels as an Index of Placental FunctionNew England Journal of Medicine, 1969