Notes on determinations of nitrate in plant material.

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Abstract
The determination of nitrate in extracts of plants including grass, maize, sugarbeet leaves, lucerne, peas, cucumber, radish and lettuce by 3 different methods was tested. The methods based on nitration of O'-xylenol and dialysis of plant extracts and reduction of nitrate to nitrite ions by a Cu-Cd reductor were equally satisfactory. No influence of the extraction ratio was found. Extraction of plant material with water or electrolyte solution gave comparable results. Direct potentiometry gave too high results at lower nitrate ion levels. This phenomenon depends on the nature of plant material analysed and is apparently caused by organic anions. For spinach, the electrode can be used when the nitrate content is about 300 mmol nitrate/kg DM or higher. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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