Glyceraldehyde‐3‐phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit
- 26 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (1) , 48-52
- https://doi.org/10.1016/j.febslet.2004.11.045
Abstract
RNA polymerase II (pol II) purified from the fission yeast Schizosaccharomyces pombe was previously reported to be associated with the general transcription factor TFIIF and the C-terminal domain phosphatase Fcp1, as well as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which has recently been implicated in transcriptional activation in human cells. Here, we provide evidence that the Rpb7 subunit of pol II interacts with GAPDH. Two-hybrid screen identified GAPDH as an Rpb7-binding protein. In addition, GAPDH was affinity-purified from S. pombe extract by using an Rpb4/Rpb7-coupled column. We also identified actin as a pol II-associated protein and revealed the interaction between actin and Rpb7.Keywords
This publication has 31 references indexed in Scilit:
- ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling ComplexScience, 2004
- S Phase Activation of the Histone H2B Promoter by OCA-S, a Coactivator Complex that Contains GAPDH as a Key ComponentCell, 2003
- The nucleoskeleton: lamins and actin are major players in essential nuclear functionsCurrent Opinion in Cell Biology, 2003
- Nuclear Actin and Actin-Related Proteins in Chromatin RemodelingAnnual Review of Biochemistry, 2002
- Cyclase‐associated proteins: CAPacity for linking signal transduction and actin polymerizationThe FASEB Journal, 2002
- Interactions of Elongation Factor 1α with F-Actin and β-Actin mRNA: Implications for Anchoring mRNA in Cell ProtrusionsMolecular Biology of the Cell, 2002
- Deletion of the RNA Polymerase Subunit RPB4 Acts as a Global, Not Stress-specific, Shut-off Switch for RNA Polymerase II Transcription at High TemperaturesPublished by Elsevier ,2001
- Rpb4, a Non-essential Subunit of Core RNA Polymerase II ofSaccharomyces cerevisiae Is Important for Activated Transcription of a Subset of GenesPublished by Elsevier ,2001
- Dissociable Rpb4-Rpb7 Subassembly of RNA Polymerase II Binds to Single-strand Nucleic Acid and Mediates a Post-recruitment Step in Transcription InitiationJournal of Biological Chemistry, 2001
- Glycolytic enzymes as DNA binding proteinsInternational Journal of Biochemistry, 1993