Autoradiographic detection of the earliest stage of [3H]‐uridine incorporation into the cow embryo

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Abstract
Cow embryos, obtained from superovulated heifers on days 3 and 4 after oestrus, were cultured for 20 min in Ménézo's complete culture medium (B2), enriched with 200 microCi/ml of 5‐[3H]‐uridine. Semi‐thin Epon sections of this material were investigated by autoradiography for sites of RNA synthesis. It was found that 5‐[3H]‐uridine was incorporated into the nucleoplasm and nucleoli only at the end of the 8‐cell stage. This suggested that synthesis of hnRNA and rRNA occurred from this stage onwards. Ultrastructural studies were performed on these embryos as well as on other non‐incubated 4‐cell embryos recovered on day 2. The transformation of dense fibrillar primary nucleoli into functional reticulated nucleoli appeared sooner in the development of cow embryos than in other mammalian species hitherto studied and took place generally during the 8‐cell stage. An unusual step in this transformation was represented by the development of a single vacuole in nucleoli at the beginning of this stage (day 3 post‐oestrus).