Characterization of a Tombusvirus Isolated from Eggplant

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Abstract
A virus that produced mottling and malformation of leaves, and stunting of growth was isolated from eggplant (S. melongena) growing in the Jiyeh area south of Beirut, Lebanon. Results of morphological, serological and biophysical analyses and observation of cytopathic effects enabled identification of the virus as a tombusvirus that is provisionally named eggplant mottled crinkle virus. It is differentiated from other tombusviruses by distinctive serological and electrophoretic properties and host range. The double antibody sandwich form of ELISA (enzyme-linked immunosorbent assay), but not the Derrick and decoration immunoelectron microscopy methods, had a rather narrow specificity with tombusviruses.