In vitro Induction of Cartilage in Mouse Somite Mesoderm by Embryonic Spinal Cord

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Abstract
Somites 5-12 from 9-day embryo mice ranging in stage from 12-24 somites (28 cases) fail to produce cartilage in Carrel flask culture or to grow and differentiate on subsequent implantation into the eye of adult males. Similar somites combined in culture with 9-day-embryo spinal cord lateral hemi-sections (29 cases) produce cartilage rods and nodules in culture, and bone, hematopoietic tissue, adipose tissue, and occasional lymphoid masses on implantation into the eye. The data are consonant with the suggestion of an inductive relation between the spinal cord and somites in the formation of axial cartilage. They further illustrate the utility of the in vitro method for study of induction and tissue interaction in mammals.