Citrate Metabolic Enzymes in Alloxan Diabetes

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Abstract
The purpose of this investigation was to study the cause(s) of increased levels of citrate in tissues and body fluids in experimental diabetes. Activity of some of the citrate metabolizing enzymes in a few tissues was determined. Citrate condensing enzyme (CCE) activity was found elevated in the “intact” diabetic rat liver homogenate. However, when the homogenate was completely broken by sonication the CCE activity was markedly elevated and the difference in activity between the diabetic and normal rat disappeared. The reason for this difference in CCE activity of sonicated and intact homogenates could be due to a decrease in the permeability of acetyl CoA into the liver mitochondria. In the diabetic rat skeletal muscle homogenate the CCE activity was markedly lower than that of the homogenate from normal rat. In the kidney, heart and brain homogenates no difference was noted.

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