Rapid Method for Isolation of Unesterified Sterols and Its Application to Detection of Milk Fat Adulteration with Vegetable Oils

Open Access
Publisher Website
Google Scholar
Abstract
Unesterified sterols were isolated from 900 mg samples of fat by using a column of digitonin impregnated on Celite 545 (Schwartz et al. 1967. J. Lipid Res. 8:54), and then eluting the sterols with dimethylsulfoxide. The sterols were extracted from the dimethylsulfoxide with hexane-ben-zene mixtures in a condition suitable for analysis by gas-liquid chromatography. The addition of 1% corn, cottonseed, soybean, or peanut oil could be seaily detected by the presence of [beta]-sitosterol. Coconut and safflower oil adulteration were detectable at the 2% level. Because of small amounts of [beta]-sitosterol present in commercial emulsifiers, ice cream fat adulteration with corn, cottonseed, soybean, or peanut oil would be detectable at the 2 to 2.5% level, while coconut and safflower oil adulteration would be detected at the 4 to 5% level.

This publication has 3 references indexed in Scilit: