Purification of a cytolytic factor from purple fluid of a sea hare
Open Access
- 17 March 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 198 (1) , 25-28
- https://doi.org/10.1016/0014-5793(86)81177-2
Abstract
A cytolytic factor inducing tumor lysis was purified approx. 1000‐fold from purple fluid of the sea hare Aplysia kurodai. The purified factor appeared homogeneous on polyacrylamide gel electrophoresis and consisted of a single polypeptide chain of 60 kDa. The cytolytic factor was half‐maximally active at 3–25 ng protein/ml and lysed all tumor cells tested, but not normal cells.Keywords
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