Activity and Isoenzyme Pattern of Lactate Dehydrogenase in Neurons and Astroblasts Cultured from Brains of Chick Embryos

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Abstract
Primary cultures of neurons and glial cells (astroblasts) prepared from brains of 8 day old and 15 day old chick embryos, respectively, were grown for periods between 3-19 days. Specific activity of lactate dehydrogenase (LDH) increased in both types of cultures as a function of time and was always significantly higher in glial cells than in neurons. Glial cell extracts contained predominantly the anaerobic isoenzymatic form of LDH (LDH-M4). This pattern did not change over a period of 19 days. Cultured neurons contained predominantly the aerobic isoenzymatic form LDH-H4. There was a progressive appearance of all other isoenzymes over an 8 day period. A different energy metabolism in neurons and glia apparently exists.
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