Purification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzae
Open Access
- 1 February 1973
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 33 (1) , 192-200
- https://doi.org/10.1111/j.1432-1033.1973.tb02669.x
Abstract
A simple scheme has been devised for the purification of Aspergillus nuclease S1 from commercially available crude amylase powder. Five purification steps yield 90% pure nuclease with an overall yield of 27%. The enzyme is shown to be absolutely specific for single‐stranded nucleic acids. It degrades both DNA and RNA. The nuclease exhibits a sharp pH optimum at pH 4.2 and is active in low concentrations of dodecylsulfate. Aspergillus nuclease S1 is a metalloprotein of molecular weight 32000. The utility of this nuclease for measuring annealing of labeled DNA is discussed.Keywords
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