Expression and Characterization of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein G (gG) by Recombinant Vaccinia Virus: Neutralization of HSV-1 Infectivity with Anti-gG Antibody
- 1 October 1987
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 68 (10) , 2587-2598
- https://doi.org/10.1099/0022-1317-68-10-2587
Abstract
Summary A recombinant vaccinia virus expressing herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) has been constructed. Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. wt. that were recognized by anti-HSV-1 sera. Rabbits and mice vaccinated with the live recombinant virus produced antibodies that recognized 48K, 57K and 61K mol. wt. proteins in HSV-1-infected cells. The gG polypeptides were present on cytoplasmic and nuclear membranes during infection with both HSV-1 and recombinant vaccinia virus gG-VAC. The 57K and 61K mol. wt. gG polypeptides were present in purified HSV-1 virions and were targets for antibody-mediated complement-dependent virus neutralization.Keywords
This publication has 21 references indexed in Scilit:
- General method for production and selection of infectious vaccinia virus recombinants expressing foreign genesJournal of Virology, 1984
- Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D.Proceedings of the National Academy of Sciences, 1984
- Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein CJournal of Virology, 1983
- Herpes Simplex Virus Type-1 Glycoprotein D Gene: Nucleotide Sequence and Expression in Escherichia coliScience, 1982
- Similarities and differences in the Fc-binding glycoprotein (gE) of herpes simplex virus types 1 and 2 and tentative mapping of the viral gene for this glycoproteinJournal of Virology, 1982
- Glycoprotein gE of herpes simplex virus type 1: effects of anti-gE on virion infectivity and on virus-induced fc-binding receptorsJournal of Virology, 1982
- Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4Infection and Immunity, 1981
- A virion-associated glycoprotein essential for infectivity of herpes simplex virus type 1Virology, 1981
- Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoproteinJournal of Virology, 1979
- Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivityJournal of Virology, 1979