CTX-M-14, a Plasmid-Mediated CTX-M Type Extended-Spectrum β-Lactamase Isolated from Escherichia coli
Open Access
- 1 June 2002
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (6) , 1985-1988
- https://doi.org/10.1128/aac.46.6.1985-1988.2002
Abstract
Four Escherichia coli isolates harboring CTX-M-14, with a single Ala231→Val substitution compared to CTX-M-9, had three different ribotypes. Cefotaxime resistance was plasmid encoded and conjugatively transferable. Three isolates had the same plasmid restriction enzyme digestion profile, suggesting clonal spread of a resistant plasmid. A high k cat / K m value for cefotaxime (20.3 μM −1 s −1 ) but low values for ceftazidime and aztreonam (−1 s −1 ) were observed in hydrolysis assays, indicating resistance to cefotaxime (MIC ⩾ 64 μg/ml) but susceptibility to ceftazidime (MIC ⩽ 2 μg/ml).Keywords
This publication has 24 references indexed in Scilit:
- Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from KenyaAntimicrobial Agents and Chemotherapy, 2001
- Cloning and Sequence of the Gene Encoding a Novel Cefotaxime-Hydrolyzing β-Lactamase (CTX-M-9) from Escherichia coli in SpainAntimicrobial Agents and Chemotherapy, 2000
- A Novel CTX-M β-Lactamase (CTX-M-8) in Cefotaxime-Resistant Enterobacteriaceae Isolated in BrazilAntimicrobial Agents and Chemotherapy, 2000
- CTX-M-type β-lactamases: an emerging group of extended-spectrum enzymesInternational Journal of Antimicrobial Agents, 2000
- Two novel plasmid-mediated cefotaxime-hydrolyzing β-lactamases (CTX-M-5 and CTX-M-6) fromSalmonella typhimuriumFEMS Microbiology Letters, 1998
- Extended-spectrum and inhibitor-resistant TEM-type beta-lactamases: mutations, specificity, and three-dimensional structureAntimicrobial Agents and Chemotherapy, 1995
- Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coliAntimicrobial Agents and Chemotherapy, 1995
- Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum β-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytocaBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- A new plasmidic cefotaximase from patients infected withSalmonella typhimuriumInfection, 1992
- Identification of -Lactamases by Analytical Isoelectric Focusing: Correlation with Bacterial TaxonomyJournal of General Microbiology, 1976