The use of flurescent dyes to measure membrane potentials: A response
- 1 August 1982
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 112 (2) , 302-305
- https://doi.org/10.1002/jcp.1041120222
Abstract
The use of fluorescent cyanine dyes to estimate membrane potential in cell suspensions has been considered. Several problems related to the application of the dyes have been reviewed. These problems include: (1) alteration of the membrane potential (Em) and factors involved in establishing Em by the dyes themselves, (2) the effect of altered energy metabolism on the fluorescent response of the dyes and on Em, and (3) calibration of dye fluorescence. Recent reports that advocate the use of the fluorescent dyes are misleading.Keywords
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