A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution
Open Access
- 15 September 1998
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 26 (18) , 4304-4305
- https://doi.org/10.1093/nar/26.18.4304
Abstract
Quantitative determination of dissociation constants for DNA-protein complexes will help clarify the molecular mechanisms of transcription, replication and DNA repair. A practical capillary electrophoresis mobility shift assay (CEMSA) for protein—DNA affinities free in solution is presented. The method is fast and simple, precise and general. The speed (Kd = 35 ± 4 nM) to demonstrate the utility of the method.Keywords
This publication has 10 references indexed in Scilit:
- Design and optimization of a capillary electrophoretic mobility shift assay involving trp repressor-DNA complexesJournal of Chromatography B: Biomedical Sciences and Applications, 1996
- DNA-protein binding assays from a single sea urchin egg: a high-sensitivity capillary electrophoresis method.Proceedings of the National Academy of Sciences, 1996
- Coupled Folding and Site-Specific Binding of the GCN4-bZIP Transcription Factor to the AP-1 and ATF/CREB DNA Sites Studied by MicrocalorimetryBiochemistry, 1996
- Towards more measurement in biologyNature, 1994
- Molecular sequestration stabilizes CAP–DNA complexes during polyacrylamide gel electrophoresisNucleic Acids Research, 1994
- Use of gel retardation to analyze protein-nucleic acid interactions.1992
- The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted α Helices: Crystal structure of the protein-DNA complexCell, 1992
- Folding transition in the DMA-binding domain of GCN4 on specific binding to DNANature, 1990
- A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory systemNucleic Acids Research, 1981
- Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresisNucleic Acids Research, 1981