Interrelationships between the Human Alveolar Macrophage and Alpha-1-Antitrypsin
Open Access
- 1 November 1973
- journal article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 52 (11) , 2793-2799
- https://doi.org/10.1172/jci107475
Abstract
Alveolar macrophages lavaged from human lungs contain protease activity at an optimum pH of 3.0 and possibly a lesser peak of activity at pH 5.5. Protease activity measured at pH 4.1 is inhibited by purified alpha-1-antitrypsin. Fluorescent antibody studies of human alveolar macrophages showed that alpha-1-antitrypsin is present in normal alveolar macrophages. In addition, macrophages from a patient with a homozygous deficiency of alpha-1-antitrypsin exhibited less fluorescence when incubated in autologous serum than the same macrophages incubated in normal serum. Macrophages from normal subjects showed maximal fluorescence when removed from the lung and additional incubation with serum did not increase fluorescence. These results implicate the human alveolar macrophage as a possible source of an enzyme that may cause emphysema in patients deficient in alpha-1-antitrypsin. They also show that alpha-1-antitrypsin has access to the alveolus in normal subjects.Keywords
This publication has 12 references indexed in Scilit:
- An Inhibitor in Human Lung Macrophages Active Against Human Neutrophil Elastase1American Review of Respiratory Disease, 1972
- The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristicsJournal of Clinical Investigation, 1971
- Composition of alveolar liquid in the foetal lambThe Journal of Physiology, 1969
- α1-antitrypsin, an inhibitor for thrombin and plasminClinica Chimica Acta; International Journal of Clinical Chemistry, 1967
- Bronchopulmonary Lavage in Normal Subjects and Patients with Obstructive Lung DiseaseAnnals of Internal Medicine, 1967
- A Possible Experimental Approach to the Association of Hereditary 1-Antitrypsin Deficiency and Pulmonary Emphysema.Experimental Biology and Medicine, 1966
- The Plasmin Inhibitors of Human PlasmaVox Sanguinis, 1965
- Electron Microscopy of Obstructive Pulmonary EmphysemaRespiration, 1965
- Fluorescent Antibody Studies with Agents of Varicella and Herpes Zoster Propagated in vitro.Experimental Biology and Medicine, 1954
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951