Immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells.
Open Access
- 1 January 1977
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 25 (1) , 69-72
- https://doi.org/10.1177/25.1.833423
Abstract
The ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. Binding to the Z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of sheep anti-rabbit gamma globulin.This publication has 0 references indexed in Scilit: