The Synthesis of RNA in Imbibing Seed of Rape (Brassica napus) prior to the Onset of Germination: A Biochemical and Cytological Study

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Abstract
Cytological experiments with radioactive tracer molecules show that seeds of rape synthesize RNA soon after they are placed in contact with water and well before physical imbibition is complete. RNA synthesis is first detectable in peripheral cells of the embryo closest to the testa. At later times of imbibition, RNA synthesis spreads progressively to the rest of the embryo. Certain cells of the imbibed seed, such as those comprising the vascular tissue, those at the base of the petiole of the outer cotyledon, and those at the margins of the cotyledons synthesize RNA at the highest rates. Gel electrophoresis and affinity chromatography of extracted RNA indicate that the transcription of ribosomal, transfer, and poly(A)-rich RNA species are initiated at about the same time in the imbibing seed. This conclusion is supported by cytological studies, for silver graining is first detected over both nucleolus and chromatin.

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