Curie-point Pyrolysis Mass Spectrometry Applied to Characterization and Identification of Selected Bacillus Species

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Abstract
The use of pyrolysis mass spectrometry in the characterization and identification of Bacillus spp. was studied. Fifty-three strains of 4 closely related groups, B. subtilis, B. pumilus, B. licheniformis and B. amyloliquefaciens, were used in a study of both sporulated and non-sporulated cultures. Pyrolysis was carried out using a Pyromass 8-80, a novel pyrolysis mass spectrometer specifically designed for fingerprinting complex samples. The pyrolysis data obtained were analyzed using multivariate statistical techniques. All 4 groups could be differentiated using data from non-sporulated cultures, but the data from sporulated cultures did not separate B. subtilis from B. amyloliquefaciens or B. pumilus. B. licheniformis was more clearly differentiated from the other 3 spp. using these data. Culture maturity affected the mass spectra obtained from non-sporulated cultures.

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