Comments on the Numerical Treatment of CPC Data

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Abstract
The magnitude of the errors introduced in the calculated molecular mass averages is discussed in the case of gel permeation chromatography (CPC). It was shown, that neglecting the undetectable parts at both tails of a chromatogram where the intensity of the curve is very small, results in serious errors. For chromatograms with Gaussian shapes it was found, that if the molecular mass limits of the calculations were set at ±35 (S is the standard deviation of the curve) the numerically calculated Mn and Mm values deviated by 1 to 10% from the theoretical ones. The errors increased with increasing polydispersity and decreased as the number of data points increased. However, there was no significant difference in the results if the number of data points was greater than 20.

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