A Simplified Bacterial Spore Stain
- 1 January 1950
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 25 (3) , 153-156
- https://doi.org/10.3109/10520295009110979
Abstract
A simplification of the Schaeffer-Fulton spore stain for bacteria is presented. It is shown that omission of the heating step during staining with malachite green resulted in spore stains as good as when the heat was applied. The simplified procedure involves (1) heat fixation of the smear by 20 passages through the flame, (2) staining with saturated aqueous malachite green for 10 minutes, (3) rinsing, and (4) counterstaining with 0.25% aqueous safranin for 15 seconds. The omission of the heating step in staining has obvious advantages, particularly in the classroom.Keywords
This publication has 5 references indexed in Scilit:
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- Staining Bacteria and Yeasts with Acid DyesStain Technology, 1941
- A Simplified Method of Staining EndosporesScience, 1933
- Wright's as A Differential Spore StainStain Technology, 1928
- A Safe Spore Stain for Class UseStain Technology, 1926