A LIPOPROTEIN AS A GROWTH FACTOR FOR CERTAIN PLEUROPNEUMONIALIKE ORGANISMS

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Abstract
Chemical and biological studies of the properties and constituents of the purified protein growth factor for pleuropneumonialike organisms reveal it to be a lipoprotein similar to or a degradation product of alpha-1-lipoprotein found in mammalian sera. The lipoprotein contains bound cholesterol and phospholipid. Employing a lipid-free medium, it can be replaced with the lipid-free protein, cholesteryl laurate, and lecithin of animal origin. Large amounts of bovine serum albumin and beta-lactoglobulin from milk also will support optimal growth indicating that the protein moiety is not greatly specific. A method of preparing the purified fraction BII from bovine blood serum also is described.