20S proteasome from LMP7 knock out mice reveals altered proteolytic activities and cleavage site preferences
- 25 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 383 (1-2) , 109-113
- https://doi.org/10.1016/0014-5793(96)00110-x
Abstract
20S proteasomes of tissues from LMP7 knock out mice which show reduced MHC class I restricted antigen presentation were analyzed with regard to their subunit composition, peptide hydrolyzing activity and their ability to cleave a synthetic 25-mer polypeptide. LMP7 deficiency results in an enhanced incorporation of subunit MB1 and in a 2–3.8-fold increase in V max for the Suc-LLVY-MCA hydrolyzing activity. Since LMP7 deficiency also affects the cleavage site preference of 20S proteasomes the reduced MHC class I antigen presentation of LMP7 knock out mice is most likely due to an impairment in peptide generation.Keywords
This publication has 25 references indexed in Scilit:
- Incorporation of major histocompatibility complex – encoded subunits LMP2 and LMP7 changes the quality of the 20S proteasome polypeptide processing products independent of interferon‐γEuropean Journal of Immunology, 1995
- LMP2+ proteasomes are required for the presentation of specific antigens to cytotoxic T lymphocytesCurrent Biology, 1995
- Altered peptidase and viral-specific T cell response in LMP2 mutant miceImmunity, 1994
- Proteasome components with reciprocal expression to that of the MHC-encoded LMP proteinsCurrent Biology, 1994
- MHC Class I Expression in Mice Lacking the Proteasome Subunit LMP-7Science, 1994
- Replacement of proteasome subunits X and Y by LMP7 and LMP2 induced by interferon‐γ for acquirement of the functional diversity responsible for antigen processingFEBS Letters, 1994
- Proteasome and cell cycleFEBS Letters, 1993
- The major‐histocompatibility‐complex‐encoded β‐type proteasome subunits LMP2 and LMP7European Journal of Biochemistry, 1993
- Proteasome subunits encoded in the MHC are not generally required for the processing of peptides bound by MHC class I moleculesNature, 1992
- A pentapeptide as minimal antigenic determinant for MHC class I-restricted T lymphocytesNature, 1989