ISOLATION OF CARCINOGEN-INDUCED DIPLOID RAT HEPATOCYTES BY CENTRIFUGAL ELUTRIATION

  • 1 September 1986
    • journal article
    • research article
    • Vol. 46  (9) , 4732-4737
Abstract
The majority of hepatocytes isolated from rats treated with carcinogens (diethylnitrosamine plus 2-acetylaminofluorene) were found to be diploid, whereas most of the hepatocytes from normal rats are tetraploid. The carcinogen-induced diploid hepatocytes were only one-half the size (protein content) of the tetraploid hepatocytes, and could therefore be separated from the latter by centrifugal elutriation The elutriation technique thus makes it possible to isolate a relatively pure fraction of carcinogen-induced cells. The diploid cells had the same liver-specific enzymatic and functional properties as the tetraploid cells and were thus undoubtedly of hepatocytic origin.