gp 140, the C3d/EBV receptor (CR2), is phosphorylated upon in vitro activation of human peripheral B lymphocytes
- 3 March 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 197 (1-2) , 353-356
- https://doi.org/10.1016/0014-5793(86)80356-8
Abstract
Gp 140, the C3d/EBV receptor (CR2), is a specific marker of human B lymphocytes. Very recent data suggest that CR2 is a membrane site involved in early B cell activation. These properties of CR2 led us to analyze the molecular events associated with gp 140. We analyzed whether in some conditions of B lymphocyte activation, CR2 could be phosphorylated. We have found that when highly enriched peripheral B cells were cultured for 48 h with anti‐μ Ab and/or SAC, in order to provide an optimal activating signal, phosphorylation of the CR2 was induced.Keywords
This publication has 19 references indexed in Scilit:
- Growth control of activated, synchronized murine B cells by the C3d fragment of human complementNature, 1985
- OKB7, a monoclonal antibody that reacts at or near the C3d binding site of human CR2Cellular Immunology, 1985
- Analysis of gp140, a C3b‐binding membrane component present on Raji cells: a comparison with factor HEuropean Journal of Immunology, 1984
- Identification of the membrane receptor for the complement fragment C3d by means of a monoclonal antibody.The Journal of Experimental Medicine, 1983
- Differential sensitivity of human B cell subsets to activation signals delivered by anti-mu antibody and proliferative signals delivered by a monoclonal B cell growth factor.The Journal of Experimental Medicine, 1983
- Behavior of soluble human 125I‐labeled C3b, the third component of complement, after binding to human cellsEuropean Journal of Immunology, 1982
- Isolation and characterization of a C3b receptor‐like molecule from membranes of a human B lymphoblastoid cell line (Raji)FEBS Letters, 1981
- Control of interaction of spectrin and actin by phosphorylationNature, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970