INCUBATION TEMPERATURES AND RAW MILK BACTERIAL COUNTS

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Abstract
Raw milk samples were plated and incubated at seven temperatures (2, 10, 20, 27, 30, 33 and 37 C). Lowest counts were obtained at 37 C. Plates at 33 C showed higher counts than at 37 C but were significantly lower than the other temperatures. The 10, 20, 27 and 30 C incubated plates were not statistically different in counts. When 32 C was substituted for 33 C, there was a significant difference (lower) only in the 37 C count. Colonies from 2, 20, 27, 33 and 37 C plates were picked and stabbed into plates subsequently incubated at these temperatures. All colonies developed best at 20 and 27 C. Evidence is presented that psychrophilic counts can be indirectly determined as the difference between counts at 27 C and 37 C.

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