Studies of Human Diploid Fibroblast Growth. I. Responses of Normal and Hypopituitary Cells to Fibroblast Growth Factor, Insulin, and Serum
- 1 October 1976
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 43 (4) , 721-729
- https://doi.org/10.1210/jcem-43-4-721
Abstract
We have assayed the growth stimulating activity of bovine insulin, fibroblast growth factor (FGF), and fetal bovine serum (FBS) in diploid human fibroblasts from normal and idiopathichypopituitary donors. All three factors stimulated DNA synthesis in cells arrested by serum starvation. FGF was active at concentrations as low as 5 ng/ml with maximum effect at 100 ng/ml. FGF stimualted DNA synthesis at lower concentrations than did insulin and also produced a greater maximum response. Only serum was capable of supporting cell division and growth, but FGF accellerated this growth rate when it was added to serum-containing medium. Hydrocortisone, actinomycin D, and cycloheximide inhibit FGF stimulation. There was no significant difference between fibroblasts from normal and hypopituitary donors.Keywords
This publication has 2 references indexed in Scilit:
- Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts.The Journal of cell biology, 1975
- Clonal growth of primary human cell culturesExperimental Cell Research, 1964