A comparison of DNA binding proteins from normal and virus‐transformed mouse cells

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Abstract
Proteins from normal and virally transformed mouse cells, capable of binding to calf thymus DNA, were isolated and compared by DNA cellulose chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis. In accordance with previous studies (Salas and Green, 1971; Tsai and Green, 1973), two major proteins, P6 and P8, and two minor proteins, P3 and PS, were found in normal fibroblasts. Extracts prepared from log phase 3T6‐MSV (RLV) synthesized increased amounts of P3, P5, and P6, while SV‐3T3 cells grown under identical conditions showed an increased level of P3 protein. Non‐producer 3T3 cells carrying a defective murine sarcoma virus genome grown under conditions of serum deprivation produced normal levels of P5, P6, and P8 proteins whereas these proteins were present in low amounts in 3T3 cells grown in a similar fashion.