INVITRO STUDY OF ATTACHMENT TO HUMAN ENTEROCYTES IN ESCHERICHIA-COLI STRAINS ISOLATED FROM INFANTS WITH DIARRHEAL DISEASE

  • 1 January 1981
    • journal article
    • research article
    • Vol. B132  (1) , 57-67
Abstract
There were 104 E. coli strains isolated from feces of 26 infants with diarrheal diseases and of 29 infants with non-diarrheal diseases tested for their ability to adhere to intestinal villi of the human small intestine. The hemagglutination (HA) type (with human, bovine, chicken and guinea-pig erythrocytes) and the agglutination of adhering strains by anti-colonization factor antigen I (CFA/I) serum and by anti-CFA/II serum were determined. Seventeen strains adhered to the brush border of the human small intestine. All these strains were isolated from infants with diarrhea; among these strains, only 2 possessed 1 CFA. One of them exhibited HA type 1 and was agglutinated by the anti-CFA/I serum; the 2nd exhibited HA type II and was agglutinated by the anti-CFA/II serum. Six strains exhibiting HA type III and 9 exhibiting other HA types were not agglutinated by anti-CFA/I or CFA/II sera. There was no relationship between the adherence and the serotypes of the antibiotic resistance of these strains.