Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system
- 1 February 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 121 (3) , 495-501
- https://doi.org/10.1042/bj1210495
Abstract
1. Factors affecting aminoacyl-tRNA synthesis in vitro by cell-free preparations from bean leaves were investigated. 2. Evidence was obtained that optimum concentrations as well as correct ratios of Mg2+ and ATP are required for aminoacyl-tRNA synthesis in the bean-leaf system. 3. The results indicated that pH is a controlling factor having differential effects on the formation of individual aminoacyl-tRNA species. The possible micro-regulatory function of pH in protein synthesis in vivo is discussed with special reference to alanyl-tRNA formation. 4. Very low rates of alanine-stimulated pyrophosphate exchange were observed in the absence of tRNA. This observation is discussed relative to proposals about the mechanism of aminoacyl-tRNA synthesis.Keywords
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